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primary antibody incubation against tnf α  (Proteintech)


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    Proteintech primary antibody incubation against tnf α
    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis <t>of</t> <t>TNF-α</t> protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Primary Antibody Incubation Against Tnf α, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibody+incubation/TNF+alpha+Antibody/pmc13034476-96-0-5
    Average 96 stars, based on 1122 article reviews
    primary antibody incubation against tnf α - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Lactobacillus acidophilus alleviates slow transit constipation by modulating 5-HT pathway and gut microbial composition"

    Article Title: Lactobacillus acidophilus alleviates slow transit constipation by modulating 5-HT pathway and gut microbial composition

    Journal: Frontiers in Nutrition

    doi: 10.3389/fnut.2026.1775405

    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Immunohistochemical staining, Quantitative RT-PCR

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    Staining:

    Article Title: The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis.
    Article Snippet: All the cells were permeabilized with Perm Buffer I (BioLegend) according to manufacturer specifications. .. Then the cell populations were identified through the following sequential staining: 1) Primary antibody incubation (room temperature, 1 h): - Hepatocytes: anti-albumin (Proteintech, 16475-1-AP) - Kupffer cells: anti-F4/80 (Abcam, ab300421) - HSCs: anti-desmin (Proteintech, 16520-1-AP) 2) HBSS washes ( ×2) 3) Secondary antibody (room temperature, 1 h): - anti-Rabbit Alexa Fluor 488-conjugated IgG (Invitrogen, A-21206) 4) HBSS washes ( ×2). .. Primary HSCs were seeded on glass-bottom confocal dishes (Cellvis, D29-20-1-N) at 70%–80% confluency.

    Article Title: FDXR Upregulation by p63/p73 is a Prognostic and Therapeutic Marker of HTLV-1-Associated Adult T Cell Leukemia/Lymphoma
    Article Snippet: .. For subsequent immunofluorescent staining, cells were blocked with 1% BSA in PBS for 30 min at RT and primary antibody incubation (anti-FDXR, 1:100, Rabbit, Proteintech #15584-1-AP) was performed in a humidified chamber at 37 °C for 1h. ..

    Incubation:

    Article Title: The Regulatory Role of Iron Transporter SLC39A13 in Liver Fibrosis.
    Article Snippet: All the cells were permeabilized with Perm Buffer I (BioLegend) according to manufacturer specifications. .. Then the cell populations were identified through the following sequential staining: 1) Primary antibody incubation (room temperature, 1 h): - Hepatocytes: anti-albumin (Proteintech, 16475-1-AP) - Kupffer cells: anti-F4/80 (Abcam, ab300421) - HSCs: anti-desmin (Proteintech, 16520-1-AP) 2) HBSS washes ( ×2) 3) Secondary antibody (room temperature, 1 h): - anti-Rabbit Alexa Fluor 488-conjugated IgG (Invitrogen, A-21206) 4) HBSS washes ( ×2). .. Primary HSCs were seeded on glass-bottom confocal dishes (Cellvis, D29-20-1-N) at 70%–80% confluency.

    Article Title: FDXR Upregulation by p63/p73 is a Prognostic and Therapeutic Marker of HTLV-1-Associated Adult T Cell Leukemia/Lymphoma
    Article Snippet: .. For subsequent immunofluorescent staining, cells were blocked with 1% BSA in PBS for 30 min at RT and primary antibody incubation (anti-FDXR, 1:100, Rabbit, Proteintech #15584-1-AP) was performed in a humidified chamber at 37 °C for 1h. ..

    Article Title: Sagittaria Sagittifolia Polysaccharide Ameliorates Diabetic Retinopathy in Mice via Inhibiting TLR4-Mediated Microglial Activation.
    Article Snippet: Sagittaria sagittifolia polysaccharide (SSP) exhibits anti-inflammatory, antioxidant, lipid-regulating, and hypoglycemic properties, demonstrating therapeutic potential against diabetic retinopathy (DR).. This study aimed to investigate the intervention efficacy of SSP in DR mice and its regulatory effects on retinal microglia activation.. The type 2 diabetic mouse model was established by high-fat diet feeding combined with streptozotocin (STZ) intravenous injection.

    Article Title: HPV11 targeting PPARA regulates the autophagy to inhibit the occurrence and development of nasal inverted papilloma
    Article Snippet: .. Primary antibody incubation was carried out overnight at 4 °C with gentle shaking using the following antibodies: anti-PPARA (1:3000, #66826-1-Ig, Proteintech, Wuhan, China), anti-HPV11E6 (1:1000, #MBS448108, MyBioSource, San Diego, USA), anti-HPV11E7 (1:1000, #ab100967, Abcam, Shanghai, China), anti-LC3B (1:1000, # T55992 , Abmart, Shanghai, China), anti-P62/SQSTM1 (1:10000, #18420-1-AP, Proteintech, Wuhan, China), anti-E-cadherin (1:20000, #20874-1-AP, Proteintech, Wuhan, China), anti-N-cadherin (1:500, #22018-1-AP, Proteintech, Wuhan, China), anti-β-actin (1:20000, #66009-1-Ig, Proteintech, Wuhan, China), and anti-GAPDH (1:50000, #60004-1-Ig, Proteintech, Wuhan, China). ..

    Article Title: Association of High Tumor-Stroma Ratio with Prostate Cancer Progression: Insights from Clinical and Genomic Data
    Article Snippet: The protein samples are then separated by SDS-PAGE electrophoresis and subsequently transferred to a PVDF membrane (IPVH00010, Millipore). .. Immunoreactions are then performed through blocking, primary antibody incubation (rabbit anti-BGN, 16409-1-AP, Proteintech), washing, and secondary antibody incubation (rabbit anti-GAPDH, 80570-1-RR, Proteintech). .. Finally, the target protein was visualized using the Odyssey CLx Imaging System (LI-COR) and images were captured.

    Article Title: Splicing variants in MYRF cause partial loss of function in the retinal pigment epithelium leading to nanophthalmos
    Article Snippet: .. Primary antibody incubation occurred overnight at 4°C: TMEM98 (1:500, 14731-1-AP, Proteintech, Rosemont, IL), Phalloidin (1:400, #A12380, Thermo Fisher, Scientific, Waltham, MA). ..

    Article Title: Unveiling Xuanshen decoction: A novel approach to combat slow transit constipation
    Article Snippet: .. Primary antibody incubation (anti-Nrf2, No.: 16396-1-AP, Proteintech, Wuhan, China, 1:100; anti-c-kit, No. 18696-1-AP, Proteintech, 1:100) proceeded at 4 °C overnight, followed by 37 °C secondary antibody (1:100) incubation for 30 minutes. ..

    Article Title: Long-term variable photoperiod exposure impairs hippocampal synapse involving of the glutamate system and leads to memory deficits in male Wistar rats.
    Article Snippet: Excessive artificial light at night can induce the human circadian misalignment, potentially impairing memory consolidation and the rhythms of hippocampal clock genes.. To investigate the impact of circadian misalignment on hippocampal function, we measured various field excitatory postsynaptic potentials (fEPSP) and golgi staining in the CA1 and dentate gyrus (DG) regions in Wistar rats.. Our findings revealed that circadian misalignment resulted in a leftward shift in the input-output (I-O) curve within the CA1 region, decreased long-term potentiation (LTP), multi-time interval paired-pulse ratio (PPR), as well as dendritic spines and complexity across both CA1 and DG regions.

    Blocking Assay:

    Article Title: Sagittaria Sagittifolia Polysaccharide Ameliorates Diabetic Retinopathy in Mice via Inhibiting TLR4-Mediated Microglial Activation.
    Article Snippet: Sagittaria sagittifolia polysaccharide (SSP) exhibits anti-inflammatory, antioxidant, lipid-regulating, and hypoglycemic properties, demonstrating therapeutic potential against diabetic retinopathy (DR).. This study aimed to investigate the intervention efficacy of SSP in DR mice and its regulatory effects on retinal microglia activation.. The type 2 diabetic mouse model was established by high-fat diet feeding combined with streptozotocin (STZ) intravenous injection.

    Article Title: Association of High Tumor-Stroma Ratio with Prostate Cancer Progression: Insights from Clinical and Genomic Data
    Article Snippet: The protein samples are then separated by SDS-PAGE electrophoresis and subsequently transferred to a PVDF membrane (IPVH00010, Millipore). .. Immunoreactions are then performed through blocking, primary antibody incubation (rabbit anti-BGN, 16409-1-AP, Proteintech), washing, and secondary antibody incubation (rabbit anti-GAPDH, 80570-1-RR, Proteintech). .. Finally, the target protein was visualized using the Odyssey CLx Imaging System (LI-COR) and images were captured.

    Article Title: Long-term variable photoperiod exposure impairs hippocampal synapse involving of the glutamate system and leads to memory deficits in male Wistar rats.
    Article Snippet: Excessive artificial light at night can induce the human circadian misalignment, potentially impairing memory consolidation and the rhythms of hippocampal clock genes.. To investigate the impact of circadian misalignment on hippocampal function, we measured various field excitatory postsynaptic potentials (fEPSP) and golgi staining in the CA1 and dentate gyrus (DG) regions in Wistar rats.. Our findings revealed that circadian misalignment resulted in a leftward shift in the input-output (I-O) curve within the CA1 region, decreased long-term potentiation (LTP), multi-time interval paired-pulse ratio (PPR), as well as dendritic spines and complexity across both CA1 and DG regions.

    Polymer:

    Article Title: Sagittaria Sagittifolia Polysaccharide Ameliorates Diabetic Retinopathy in Mice via Inhibiting TLR4-Mediated Microglial Activation.
    Article Snippet: Sagittaria sagittifolia polysaccharide (SSP) exhibits anti-inflammatory, antioxidant, lipid-regulating, and hypoglycemic properties, demonstrating therapeutic potential against diabetic retinopathy (DR).. This study aimed to investigate the intervention efficacy of SSP in DR mice and its regulatory effects on retinal microglia activation.. The type 2 diabetic mouse model was established by high-fat diet feeding combined with streptozotocin (STZ) intravenous injection.

    Labeling:

    Article Title: Sagittaria Sagittifolia Polysaccharide Ameliorates Diabetic Retinopathy in Mice via Inhibiting TLR4-Mediated Microglial Activation.
    Article Snippet: Sagittaria sagittifolia polysaccharide (SSP) exhibits anti-inflammatory, antioxidant, lipid-regulating, and hypoglycemic properties, demonstrating therapeutic potential against diabetic retinopathy (DR).. This study aimed to investigate the intervention efficacy of SSP in DR mice and its regulatory effects on retinal microglia activation.. The type 2 diabetic mouse model was established by high-fat diet feeding combined with streptozotocin (STZ) intravenous injection.

    Gentle:

    Article Title: HPV11 targeting PPARA regulates the autophagy to inhibit the occurrence and development of nasal inverted papilloma
    Article Snippet: .. Primary antibody incubation was carried out overnight at 4 °C with gentle shaking using the following antibodies: anti-PPARA (1:3000, #66826-1-Ig, Proteintech, Wuhan, China), anti-HPV11E6 (1:1000, #MBS448108, MyBioSource, San Diego, USA), anti-HPV11E7 (1:1000, #ab100967, Abcam, Shanghai, China), anti-LC3B (1:1000, # T55992 , Abmart, Shanghai, China), anti-P62/SQSTM1 (1:10000, #18420-1-AP, Proteintech, Wuhan, China), anti-E-cadherin (1:20000, #20874-1-AP, Proteintech, Wuhan, China), anti-N-cadherin (1:500, #22018-1-AP, Proteintech, Wuhan, China), anti-β-actin (1:20000, #66009-1-Ig, Proteintech, Wuhan, China), and anti-GAPDH (1:50000, #60004-1-Ig, Proteintech, Wuhan, China). ..

    Electrophoresis:

    Article Title: Long-term variable photoperiod exposure impairs hippocampal synapse involving of the glutamate system and leads to memory deficits in male Wistar rats.
    Article Snippet: Excessive artificial light at night can induce the human circadian misalignment, potentially impairing memory consolidation and the rhythms of hippocampal clock genes.. To investigate the impact of circadian misalignment on hippocampal function, we measured various field excitatory postsynaptic potentials (fEPSP) and golgi staining in the CA1 and dentate gyrus (DG) regions in Wistar rats.. Our findings revealed that circadian misalignment resulted in a leftward shift in the input-output (I-O) curve within the CA1 region, decreased long-term potentiation (LTP), multi-time interval paired-pulse ratio (PPR), as well as dendritic spines and complexity across both CA1 and DG regions.

    Membrane:

    Article Title: Long-term variable photoperiod exposure impairs hippocampal synapse involving of the glutamate system and leads to memory deficits in male Wistar rats.
    Article Snippet: Excessive artificial light at night can induce the human circadian misalignment, potentially impairing memory consolidation and the rhythms of hippocampal clock genes.. To investigate the impact of circadian misalignment on hippocampal function, we measured various field excitatory postsynaptic potentials (fEPSP) and golgi staining in the CA1 and dentate gyrus (DG) regions in Wistar rats.. Our findings revealed that circadian misalignment resulted in a leftward shift in the input-output (I-O) curve within the CA1 region, decreased long-term potentiation (LTP), multi-time interval paired-pulse ratio (PPR), as well as dendritic spines and complexity across both CA1 and DG regions.



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    Image Search Results


    ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Journal: bioRxiv

    Article Title: Neural cell state modulation by PARK2 and dopaminergic neuroprotection by small molecule Parkin agonism

    doi: 10.64898/2026.04.01.715918

    Figure Lengend Snippet: ( a ) TH and DAT staining in matured iPSC-derived dopaminergic neurons used to assess the efficiency of dopaminergic neuron generation. Quantification of Tuj1-positive neurons divided by the total number of cells (DAPI: 4,6-diamidino-2-phenylindole) yielded the percentage of neuronal cells per imaging field. Scale bar = 20 µm. Additionally, the quantification of TH-positive neurons divided by the total number of neurons (number of Tuj1-positive) yielded the percentage of dopaminergic neurons per imaging field. ( b ) Parkin upregulation in healthy iPSC-derived dopaminergic neurons following 48 h of incubation with 4 µM and 8 µM FB231 or DMSO (Cont). GAPDH was used as a loading control. ( c ) FB231 (4 µM) and DMSO-treated dopaminergic neurons were harvested 36 hours after treatment for Western blot and other experiments. ( d and e ) qPCR analysis of the samples used in c . ( f ) Analysis pipeline used for detecting and quantifying organelle morphology. Scale bar = 10 µm. ( g ) Transmission electron microscopy (TEM) characterization of recombinant PFFs before and after sonication, via fibril length. ( h ) Large-field Mitotracker (Mito) and Lysotracker (Lyso) staining and colocalization in neurons previously exposed to PFFs at 1 µg/mL for 24 h, then exposed to 4µM FB231 or DMSO for 24 h. Scale bar = 20 µm.

    Article Snippet: Membranes were incubated with primary antibodies overnight at 4°C following blocking with 5% Bovine Serum Albumin (Millipore Sigma, A9647-100G) in TBS with Tween (Thermo Scientific Chemicals, J77500.K2) for 1 h. Primary antibody incubation was done overnight, while secondary antibody incubation was done at room temperature for 1 h. Membranes were imaged using ChemiDoc (Bio-Rad) and analyzed using Image Lab software (Bio-Rad).

    Techniques: Staining, Derivative Assay, Imaging, Incubation, Control, Western Blot, Transmission Assay, Electron Microscopy, Recombinant, Sonication

    Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Nutrition

    Article Title: Lactobacillus acidophilus alleviates slow transit constipation by modulating 5-HT pathway and gut microbial composition

    doi: 10.3389/fnut.2026.1775405

    Figure Lengend Snippet: Lactobacillus acidophilus modulates the expression of inflammatory cytokines. (A) Immunohistochemical analysis of TNF-α protein expression in mouse colon tissue. (B) RT-qPCR analysis of TNF-α mRNA expression in colonic tissues. (C) RT-qPCR analysis of IL-1β mRNA expression in colonic tissues. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Primary antibody incubation against TNF-α (Proteintech, Wuhan, China) (60291-1-IG) (dilution 1:1000) was performed overnight at 4 °C.

    Techniques: Expressing, Immunohistochemical staining, Quantitative RT-PCR